Design and Testing of High-Affinity Mutants of Interferon Gamma Receptor 1
نویسندگان
چکیده
منابع مشابه
Increasing Affinity of Interferon-γ Receptor 1 to Interferon-γ by Computer-Aided Design
We describe a computer-based protocol to design protein mutations increasing binding affinity between ligand and its receptor. The method was applied to mutate interferon-γ receptor 1 (IFN-γ-Rx) to increase its affinity to natural ligand IFN-γ, protein important for innate immunity. We analyzed all four available crystal structures of the IFN-γ-Rx/IFN-γ complex to identify 40 receptor residues ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2013
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2012.11.3091